Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Usage Physical State: Freeze-dried solidStorage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ConjugateHorseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Images & ReferencesThis product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
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货号 | 产品名称 | 来源 | 货号 | 产品名称 | 来源 | 货号 | 产品名称 | 来源 |
C0002001 | 5637 cells | 原发性膀胱癌2级 | C0009012 | RKO cells | 弱分化的结肠癌细胞 | C0025002 | 8505C cells | 未分化甲状腺癌 |
C0003001 | CCRF-CEM cells | 淋巴母细胞 | C0009011 | LoVo cells | Dukes C型,等级4 | C0025001 | FTC-238 cells | 甲状腺滤泡状癌 |
C0003004 | K562 cells | 胸腔积液 | C0009010 | HCT15 cells | Dukes C型 | C0025003 | K1 cells | 甲状腺乳头状癌 |
C0003006 | LP-1 cells | 胸腔积液 | C0010001 | HUTU-80 cells | 原发性膀胱癌(2级) | C0026001 | HEC-59 cells | 子宫内膜癌 |
C0003003 | MOLM-13 cells | 外周血 | C0013001 | EC-109 cells | 弱分化的鳞癌 | C0008001 | HeLa cells | 子宫颈腺 |
C0003002 | RPMI-8226 cells | B淋巴细胞 | C0013002 | HKESC-1 cells | 弱分化的鳞癌 | C0008002 | SiHa cells | 子宫鳞状细胞癌 |
C0003005 | U937 cells | 组织细胞性淋巴肉瘤 | C0013003 | OE33 cells | 食管腺癌 | C0008004 | CaSKi cells | 小肠内的转移 |
C0005004 | SJSA-1 cells | 原发性股骨原始多向肉瘤 | C0013004 | JROECL33 cells | 食管腺癌 | C0008003 | C33A cells | 宫颈癌活检组织 |
C0005003 | B103 cells | 神经母细胞瘤 | C0015001 | Hep3B cells | 8岁黑人瘤组织 | C0018007 | INS-1 cells | 胰腺组织 |
C0005001 | LN-319 cells | 左恶性胶瘤 | C0015002 | HepG2 cells | 15岁阿根廷男瘤组织 | C0018008 | MIN-6 cells | 胰腺组织 |
C0005004 | SH-Sy5y cells | 骨髓转移 | C0016004 | A427 cells | 52岁白人男肺癌 | C0020003 | UACC-62 cells | 黑瘤 |
C0005002 | U87MG cells | 恶性神经胶瘤 | C0016002 | A549 cells | 可通过胞苷二磷酸胆碱途径合成磷脂 | C0005005 | SF-295 cells | 未分化的胶母细胞瘤 |
C0006004 | 4T1 cells | 无诱变剂处理的410.4肿瘤 | C0016001 | Calu3 cells | 转移性胸腔积液 | C0009016 | SNU-407 cells | 转移性的臀 |
C0006005 | JIMT-1 cells | 胸腔积液 | C0016003 | H460 cells | 转移性胸腔积液 | C0009015 | SNU-503 cells | N/A |
C0006002 | MDA-MB-231 cells | 胸腔积液 | C0017002 | A2780 cells | 未处理瘤组织 | C0009013 | LS-174T cells | 结肠腺癌,Dukes B型, |
C0006007 | SK-BR-3 cells | 胸腔积液 | C0017001 | MDAH-2774 cells | 子宫内膜卵巢癌 | C0018009 | INS-1E cells | 胰岛组织 |
C0006001 | T47D cells | 转移性胸腔积液 | C0017002 | A2780 cells | 未处理瘤组织 | C0006003 | MDA-MB-468 cells | 胸腔积液 |
C0006008 | MCF-7 cells | 胸腔积液 | C0018004 | AR-42J cells | 胰腺外分泌肿癌 | C0023002 | N87 cells | 转移性肝 |
C0009009 | Caco-2 cells | 结肠腺癌 | C0018003 | BxPc-3 cells | 褐鼠原发性胰腺癌 | C0003010 | Daudi cells | 伯奇士淋巴瘤 |
C0009007 | DLD-1 cells | 结肠腺癌,C型Duke | C0018001 | Capan-1 cells | 肝 | C0003013 | NAMALWA cells | 伯奇士淋巴瘤 |
C0009003 | HCA-7 cells | 中等分化的结肠腺癌 | C0018002 | MIA PaCa-2 cells | 未分化的胰腺癌 | C0003007 | PBL-2H3 cells | 化学诱导的嗜碱细胞 |
C0009005 | HCT-116 cells | 原发性结肠癌 | C0018005 | E3LZ10.7 cells | 转移性肝癌(临床阶段4) | C0003009 | Raji cells | 左上颌骨 |
C0009001 | SW480 cells | 原发性结直肠癌 | C0018006 | A10.7 cells | 转移性肝癌(临床阶段4) | C0003012 | RS4;11 cells | 骨髓 |
C0009004 | HT-29 cells | 结直肠腺癌 | C0019002 | PC-3 cells | 转移性的骨头 | C0003011 | SU-DHL-6 cells | 胸腔积液 |
C0009006 | LS-180 cells | Dukes B型的结直肠癌 | C0019001 | Vcap cells | 椎体转移癌 | C0016008 | NCI-H1703 cells | 抽烟者 |
C0009002 | SW620 cells | 转移性淋巴结 | C0020001 | SK-Mel-28 cells | 黑素瘤 | C0016005 | NCI-H23 cells | 未治疗肺癌 |
C0009008 | T84 cells | 转移性肺 | C0023001 | KATO III cells | 转移性胸腔积液; 锁骨和腋窝淋巴结和子宫直肠窝 | C0016009 | SW1573 cells | 肺泡细胞癌 |
C0009014 | SW48 cells | 肠溃疡肿瘤IV级 |